Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR.
PCR Systems and Consumables
|Droplet Digital Systems||Digital PCR Reagents and Assays||Digital PCR Accessories|
|QX200™ AutoDG™ Droplet Digital™
|ddPCR Supermixes||ddPCR Oils|
|For high-throughput analysis||For sensitive DNA target detection||For creating sample-in-oil droplets|
|QX200™ Droplet Digital™
PCR (ddPCR™) System
|PrimePCR™ ddPCR™ Assays||ddPCR Consumables|
|For analyzing up to 96 samples||Experimentally validated primer
|For use with the droplet generator|
|ddSEQ™ Single-Cell Isolator||Digital PCR Library
|For single-cell gene expression
|For quantifying sequencing
|For secure plate
Droplet Digital PCR Technology Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology.
|DNA sample with target sequence is partitioned into droplets|
|Target and background DNA are randomly distributed among 20,000 droplets|
|Target sequence is amplified by end-point PCR in each droplet|
|Positive droplets are counted to give precise quantification of target sequences in sample|
|Copy number variation (CNV)
Accurate CNV quantification in single wells
|Next generation sequencing
Amplification and accurate quantification of NGS libraries with validation of sequencing results
|Detection of rare sequences in cancer and disease
Detection of mutations and viral load below levels detectable by current test
|Gene expression analysis
Detection of rare mRNAs and miRNAs with rapid turnover
|Pathogen detection and microbiome analysis
Viral load and pathogen detection in organ transplants, soil, and food
|Single cell analysis
High sensitivity make it a robust tool for single-cell analyses
Download the Droplet Digital PCR Applications Guidefor in-depth information about setting up experiments.
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